Enzymatic Hydrolysis of Silk Fibroin Peptide for Hemorrhage Control

Abstract

Effective hemorrhage control becomes increasingly significant in today’s military and civilian trauma, while the topical hemostats currently available in market still have various disadvantages. In this study, the silk fibroin peptide (SFP) was prepared through alcalase hydrolysis of silk fibroin. The result of thromboelastographic (TEG) method showed that the SFP can significantly strengthen the coagulation in blood. The results of APTT measurements reflected the acceleration of activation of intrinsic pathway. In the murine hepatic injury model, the blood loss and the bleeding time significantly reduced with the use of SFP as compared with the equal masses of gauze. Based on these results, it can be suggested that the developed SFP has the excellent hemostatic effect, caused no observable tissue damage at the site of application and would be a promising material in clinical hemostatic application.