Immobilization of Horseradish Peroxidase on ModifiedElectro -spunNanofibrous Membrane for DESRemoval

Ngabonziza Jean Robert, Jianmei YUAN, Ran XU

Abstract


Polyacrylontrile (PAN)/Poly (m-phenylene isophthalamide) PMIA electrospun nanofibrous membranes were hydrophobic ones that were not suitable for the removal of organic pollutants in aquatic environment. We employed an easy and simple method to convert the hydrophobic membranes into hydrophilic ones. Horseradish peroxidase (HRP) was immobilized on the modified PAN/PMIA/Cu membrane and the maximum enzyme loading was approximately 900 mg/g. Compared with free HRP, the pH, temperature, operational and storage stability were greatly improved after immobilization. Diethylstilbestrol (DES) removal experiment showed that the immobilized HRP and free HRP showed a similar removal efficiency (87% and 93%, respectively). However, the immobilized HRP has an excellent reusability.

Keywords


Diethylstilbestrol, Horseradish peroxidase immobilization, Nanofibrous membrane


DOI
10.12783/dtmse/msce2016/10489

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