Effect of (Huai) Rehmanniae Radix Praeparata Polysaccharides on Immune Organs of Rats with Qi and Blood Both Deficiency

Abstract

Purpose: Investigate the protective effect of (Huai) Rehmanniae Radix Praeparata poly-saccharides on immune organs of rats with qi and blood both deficiency. Methods: Sixty rats were randomly divided into blank group, angelica blood tonic oral liquid group, and a large, medium and small dose of Rehmanniae Radix Praeparata polysaccharides groups. In addition to the blank group, the model of qi and blood both deficiency in rats was established by tail bloodletting and intraperitoneal injection of cyclophosphamide. After the final administration for 2h, the rats were sacrificed with decervical vertebra, and the thymus and spleen were taken for pathological sections. The thickness of thymus cortex and the number of lymphocytes, the size of splenic nodules and the number of lymphocytes were observed under the microscope. By measuring the widest and narrowest areas of the thymic cortex and averaging its thickness, count the number of lymphocytes loaded on the micrometer scale. The number of lymphocytes pressed on both sides of the line was calculated by taking the central artery of the spleen as the midpoint, the mean is the number of lymphocytes in the surrounding lymphatic sheath of a central arteriole. The other three number of lymphocytes in the surrounding lymphatic sheath of the central splenic artery was calculated by the same method, taking the mean as the number of lymphocytes. Results: In the model group, thymus thickness and spleen nodules decreased significantly, the numbers of lymphocytes in thymic cortex and spleen decreased significantly (P<0.01). Rehmanniae Radix Praeparata polysaccharides groups with different dose could increase the thickness of thymic cortex, enlarge the splenic nodule and increase the number of lymphocytes in thymic cortex and spleen (P<0.01 or P<0.05). Conclusion: (Huai) Rehmanniae Radix Praeparata polysaccharides can improve the pathological changes of immune organs in rats with qi and blood both deficiency.