Screening and Molecular Identification of a Bacterial Strain with Higher Lipase Productivity

Abstract

The objective of this study was to screen lipase-producing strain from rich oil soil, then complete molecular identification and some culture characteristics of the isolated strain. One out of 10 strains isolated from different samples was obtained with higher lipase production, which was identified as Proteusbacillus vulgaris according to its 16S rDNA sequence and analysis phenotypic characterization together with its morphological and physiological characterization, it was nominated as Proteusbacillus vulgaris Cz-6. Some conditions of Proteusbacillus vulgaris Cz-6 for lipase production were studied in detail. The results showed that the optimal carbon source and nitrogen source in culture medium were glucose and peptone. The optimal initial pH and culture temperature were pH 5.5 and 37°C respectively. Under these conditions, after cultivated for 72 h, the measured strain’s enzyme activity grew up to 15.36 U•m L-1, which increased by 12% than the initial one before optimization.