Purification of Factor VIII From Plasma by Q SepharoseTM Fast Flow Column

PENG-BO HOU, QING-HUA LIU, XIAO-NING JIA, LIN FU, MIN LIU, SHOU-DONG GUO, WEN-GANG SONG

Abstract


This article reported a method to prepare high quality of factor VIII by Q SepharoseTM Fast Flow. Results indicated that the specific activity of the FVIII obtained was varying between 55 and 103 IU/mg of protein. This means that this procedure could provide at least 458- to 858-fold purer FVIII compared with the original plasma with a recovery rate approximately 71.2 %. The procedure is easily applicable to large-scale production of FVIII with acceptable recovery.

Keywords


Haemophilia, Factor VIII activity, Cryoprecipitation.


DOI
10.12783/dtbh/icmsb2017/17954

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